A simple, precise, accurate, rapid and economical High Performance Thin Layer chromatographic method has been developed and validated for the estimation of Rosuvastatin calcium (ROSU) and Aspirin (ASP) simultaneously in combined capsule dosage forms. The mobile phase used was a mixture of Methanol: Buffer, 0.02M (80:20 v/v, pH 4.0). The detection was carried out at 258 nm. The method was validated in terms of linearity, accuracy, precision, LOD, LOQ, range and robustness. The calibration curve was found to be linear between 8-12 µg/ml for ROSU and 60-90 µg/ml for ASP. The limit of detection and the limit of quantification for ROSU were found to be 0.080µg/ml and 0.244µg/ml respectively, and for ASP, 0.024µg/ml and 0.074µg/ml respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.
Loading....